《植物生理学报》 2014, 50(4): 477-482

MeJA熏蒸叶片对新疆沙冬青根细胞跨膜离子流及抗盐性的影响

刘蕾^1,2, 高海波³, 沈应柏^1,2,*

北京林业大学¹生物科学与技术学院, ²林木育种国家工程实验室, 北京100083; ³临沂大学生命科学学院, 山东临沂276005

通信作者：沈应柏；E-mail: ybshen@bjfu.edu.cn；Tel: 010-62336563

摘　要：

土壤盐渍化是植物生长的主要逆境之一。茉莉酸类物质与植物抗逆性密切相关。以国家二级保护植物新疆沙冬青为实验材料, 利用非损伤微测技术, 从动态离子流转运的角度研究了MeJA预处理后NaCl处理对新疆沙冬青根部K⁺、H⁺、Na⁺流的影响。结果表明, MeJA预处理的新疆沙冬青经盐胁迫后根冠区瞬时K⁺外流低于未经MeJA预处理的对照植株, H⁺外流强度大于对照植株; 新疆沙冬青根尖区域(0~3 000 μm)经MeJA和NaCl (50 mmol·L^-1, 24 h)预处理, 解除NaCl胁迫后观察到Na⁺外流和H⁺内流, 流速高于对照植株。表明适当浓度MeJA预处理能有效提高新疆沙冬青苗期抗盐性。

关键词：盐胁迫; H⁺-ATPase; MeJA; 非损伤微测技术

收稿：2013-12-06   修定：2014-01-10

资助：国家自然科学基金(31270655和30871727)、国家863计划(2011AA100200)和高等学校博士学科点专项科研基金(20090014110014)。

Effects of MeJA Fumigation to Leaves on Ion Flux in Apical Region of Ammopiptanthus nanus and Salt Resistance

LIU Lei^1,2, GAO Hai-Bo³, SHEN Ying-Bai^1,2,*

¹College of Biological Sciences and Technology, ²National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; ³College of Life Sciences, Linyi University, Linyi, Shandong 276005, China

Corresponding author: SHEN Ying-Bai; E-mail: ybshen@bjfu.edu.cn; Tel: 010-62336563

Abstract:

Soil salinization is one of serious factors restricting the expansion of agriculture and forestry around the world. Jasmonic acid is closely associated with plants on salt tolerance. Ion fluxes were studied in the roots of Ammopiptanthus nanus, a second class national-level protected plant, by using non-invasive micro-test technique. The result showed that the plants with MeJA pretreatment have lower efflux of K+ and higher efflux of H+ than the plants without pretreatment under the stress of NaCl in the root cap. After short-term salt stress (50 mmol·L-1 NaCl, 24 h), the plants with MeJA pretreatment have higher Na+ efflux and H+ influx than the plants without pretreatment in root apex. The results indicate that suitable concentration of MeJA pretreatment can increase salt stress resistance of A. nanus at seedling stage.

Key words: salt stress; H+-ATPase; MeJA; non-invasive micro-test technique